肉品中肠毒素性大肠杆菌和肠沙门菌多重PCR检测方法的建立

Establishment of PCR Detection for Enterotoxingenic Escherichia coli and Salmonella enteria in Meat

利用肠毒索性大肠杆菌（ETEC）的LT-A、ST-1毒素基因和肠沙门菌的iroB基因设计3对引物,建立能同时检测ETEC和肠沙门菌的多重PCR方法.结果：多重PCR扩增出了预计的PCR产物,本方法的特异性为100％、灵敏度达10102 CFU/mL,用建立的多重PCR方法对30份临床可疑腹泻样品进行检测并与生化试验比较,结果两种方法检出大肠杆菌和沙门菌的阳性符合率达100％.

The objective of this study was to use LT-A, ST-1 enterotoxigenic genes of ETEC and iroB gene of Salmonella ente-ria to design three different pairs of oligonucleotide primers in order to establish a multiplex PCR detection method which both si-muhaneously detected ETEC and Salmonella enteria. Results：The expected PCR products were amplified, respectively. 30 meatspecimens from suspicious and diarrheal swine were examined using this muhiplux PCR and biochemical test, with the specificityreaching 100% and the sensitivity 10-llY bacteria/mho. The positive detection rates of Escherichia coli and Salmonella using twokinds of methods showed 100% conformity.