朝鲜百合离体多倍体诱导

Polyploid induction of Lilium amabile in vitro

避光条件下,用0.10%秋水仙素附加2.00%二甲亚砜诱变离体培养的朝鲜百合小鳞茎,利用组织培养的不定芽诱导技术获得了多倍体幼苗,并对其根尖染色体数目进行常规鉴定。结果表明：诱导48h效果好,变异率达到50.00%。对照植株和得到的4个变异株系进行细胞学观察后发现,对照为二倍体（2n=2x=24）,诱变出的4个变异株系细胞染色体数目分别由34-54条的不同比例构成,其中四倍体细胞染色体（2n=4x=48）占38.18%-76.47%,属于嵌合体。

Bulblets of Lilium amabile were treated by 0 .10% colchicine with 2 .00% dimethyl sulfoxide for addition in vitro without light ,and the polyploid seedlings were obtained by adventitious bud induction .For the observation of the chromosome number in polyploid ,the result of 48hourinduction leaded to a variation rate of 50 .00% .From the cytological observation with a control group of diploid （2n=2x=24）of 4 mutative strains ,the chromosome numbers after induction were in the range of 34-54 with different proportion ,and 38 .18% -76 .47% of the inducted cells were tetraploid （2n=4x=48） ,and they were chimera .