不同温度条件下抗菌素氟苯尼考在家蚕体内的药代动力学特征

Pharmacokinetic Characteristics of Antibiotic Florfenicol in Silkworm Under Different Temperatures

氟苯尼考是新一代氯霉素类抗菌素,可用于家蚕细菌病防治。针对全年各养蚕季节环境温度相差较大的情况,研究不同环境温度下氟苯尼考在家蚕体内的药代动力学特征,为不同养蚕季节的蚕病防治合理用药提供科学依据。以500μg/m L氟苯尼考药液浸渍后的桑叶给不同温度条件下饲养的家蚕5龄第5天幼虫添食,采用高效液相色谱法(HPLC)检测添食药物后不同时间蚕体血浆中的药物浓度,经药理学计算软件DAS 2.0分析显示:给药后蚕体血浆中氟苯尼考的浓度不断上升,30℃处理区与24℃处理区的峰值分别出现在给药后3 h和4 h;添食3 h后停止给药,30℃处理区蚕体中的血药浓度逐渐下降,至停药1.5 h后低于24℃处理区。试验结果表明,氟苯尼考在家蚕体内不同时间的血药浓度变化受环境温度影响,高温环境下蚕体对药物的吸收快,但消除也快,故在夏秋蚕季高温环境下使用氟苯尼考防治家蚕细菌病,需要适当增加添食药物的浓度。

Florfenicol, one of the new generation chloramphenicol antibiotics, can be used for the prevention and control of silkworm （Bombyx mori） bacterial disease. According to the great temperature difference among different silkworm rearing seasons in a year, the pharmacokinetic characteristics of florfenicol in silkworm under different environmental temperatures was studied to provide scientific basis for the rational use of drugs to control silkworm disease in different seasons. After administration of 500 pg/mL fiorfenicol to day 5 silkworm larvae of the 5th instar reared under different temperatures, the concentrations of florfenicol in silkworm plasma were determined by high performance liquid chromatography （HPLC） at different time points, and the data were analyzed with the pharmacokinetic calculation software DAS 2.0. The results indicated that the concentration of florfenicol in silkworm plasma was increased constantly after drug administration, and the peak value appeared at 3 h and 4 h after drug delivery at 30 ℃ and 24 ℃ respectively. After continuous administration of florfenicol treated leaves for 3 d, the concentration of florfenicol in silkworm plasma of the treatment group florfenicol to prevent silkworm bacterial disease in summer and autumn under high temperature condition.