碱溶酸沉法提取黑木耳蛋白质的工艺优化

Optimization of the Alkali Solution and Acid Precipitation Method to Extract Protein from Auricularia auricula

主要以黑木耳为原料,利用碱溶酸沉的方法提取其蛋白质成分。以蛋白质提取率为参考指标,来研究提取p H、料液比、提取温度和提取时间对黑木耳蛋白质提取率的影响。通过单因素试验确定各因素最佳的提取工艺参数为:提取p H 10.5,料液比1∶80(g/m L),提取时间2.0 h,提取温度35℃。在此基础上,通过多因素重复试验对提取条件进行优化,确定各影响因素的最优组合为:提取时间2.0 h,提取p H 10.0,提取温度40℃,料液比1∶90(g/m L)。在此条件下,黑木耳蛋白质的提取率可达64.80%。并测定了黑木耳蛋白质的等电点在p H 3.0附近。

The extraction of protein from Auricularia auricula,using alkali-solution and acid-isolation method,was investigated.The extraction rate of protein was regarded as the reference.Altogether four factors（extract pH,flour/solvent ratio,extract temperature and extract time） were examined for Auricularia auricula protein extraction It was found that the optimum condition was that the extract pH 10.5,the flour to solvent ratio 1：80（g/mL）,extract temperature 35 ℃,extract time 2.0 h in the single-factor experiment.The extract condition was optimized by designing the repeat-multi-factors experiments.The results showed that extract time 2.0 h,pH 10.0,temperature 40 ℃,flour to solvent ratio 1：90（g/mL）.Under this condition,the extraction rate of Auricularia auricula proteincould reach to 64.80%.Determine the isoelectric point of the Auricularia auriculaprotein was close to 3.0.