海藻多糖的两种提取方法及其降糖活性比较

Extraction and Isolation of Seaweed Polysaccharide and Study on Its Hypoglycemic Activity

采用水提和醇提两种方法从海藻中提取多糖,并以胰淀粉酶为靶酶,4-硝基苯-α-D-吡喃葡萄糖苷(PNPG)为底物,测定其降糖活性,比较两种方法的提取率及提取多糖的降糖活性。试验结果表明,醇提和水提法的提取率分别6.0%和7.8%;以多糖质量浓度为12 mg/m L时的抑制率相比较,醇提多糖的抑制率为60.5%,水提多糖的抑制率为59.38%,脱蛋白醇提多糖的抑制率为41.35%;醇提多糖的IC50为2.240 mg/m L,水提多糖的IC50为2.850 mg/m L,脱蛋白醇提多糖的IC50为2.630 mg/m L。

Water and alcohol two methods was used to extract algae polysaccharide from seaweed,and study the hypoglycemic activify was studied.Seaweed polysaccharide was used as trypsin inhibitors and the effect of PNPG on trypsin was determined to identify hypoglycemic activify.The results showed that the extraction rate of polysaccharide by water and alcohol was 6.0%and 7.8%.Compared with the inhibition rate when polysaccharide concentration was 12 mg/mL,the inhibition rate of polysaccharides extracted with ethanol was 60.5%,the inhibition rate of polysaccharides extracted with water was 59.38%,the inhibition of polysaccharide extracted with de-protein alcohol was 41.35%.Polysaccharide alcohol IC50 was 2.240 mg/mL,water polysaccharide IC50 was 2.850mg/mL,alcohol extract protein from the polysaccharide IC50 was 2.630 mg/mL.