摘要
目的调查黑龙江省逊克县火山熔岩地区啮齿动物携带病原体状况。方法 2014年4-9月,采集啮齿动物样本107只,解剖取脏器肺、肾、肝、肾和膀胱样本,采用PCR方法分别扩增10种鼠传病原体的特异性核酸片段,通过基因测序进一步鉴定。结果在107份鼠肺中共检出汉城型汉坦病毒RNA核酸阳性样本5份,感染率为4.67%;107份鼠肾中共检出钩端螺旋体DNA核酸阳性样本6份,感染率为5.61%;107份鼠脾中共检出巴尔通体DNA核酸阳性样本7份,感染率为6.54%,嗜吞噬细胞无形体4份,感染率为3.74%;107份鼠肝、鼠脾中鼠疫菌、巴贝西原虫、斑点热群立克次体、恙虫病立克次体、莫氏立克次体的DNA核酸检测结果均为阴性;107份鼠膀胱中伯氏疏螺旋体的DNA核酸检测结果为阴性;在鼠体内还存在复合感染情况,感染率为2.80%。结论黑龙江省逊克县火山熔岩地区啮齿动物中存在汉坦病毒、钩端螺旋体、巴尔通体和嗜吞噬细胞无形体的感染。
Objective To investigate rodent- borne pathogens in volcano lava areas of Xunke county in Heilongjiang, China. Methods A total of 107 rodents were collected during April to September in 2014, and necropsy was conducted to acquire lung, kidney, liver, and kidney and bladder samples. All samples were amplified for the specific fragments of 10 rodent- borne pathogens by PCR, and further identified by gene sequencing. Results Five positive samples of SEO Hantavirus RNA were detected from 107 rodent' s lung tissues, infection prevalence was 4.67%; 6 positive samples of leptospirosis DNA were detected from 107 rodent's kidney tissues, infection prevalence was 5.61%; 7 positive samples of Bartonella and 4 positive samples of Anaplasma phagocytophilum were detected from 107 rodent' s spleen tissues, the prevalence of infection were 6.54% and 3.74% respectively; Detection of Yersinia pestis, Babesia, Spotted fever group rickettsia, Rickettsia tsutsugamushi, Rickettsia mooseri from rodent' s liver and spleen tissues and Borrelia burgdorferi from rodent' s Bladder was all negative. Multiple infection was found in some rodents, the infection prevalence was 2.80%. Conclusion Hantavirus, Leptospirosis, Bartonella, Anaplasma phagocytophilum exist in rodent populations that dwell in the volcano lava areas of Xunke county, Heilongjiang, China.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2015年第3期238-241,共4页
Chinese Journal of Vector Biology and Control
基金
国家质量监督检验检疫总局科研基金(2014IK047)
黑龙江出入境检验检疫局科技计划项目(2014HK007)~~