抗吡丙醚淡色库蚊对5种常用杀虫剂的交互抗性

Cross-resistance in pyriproxyfen-resistant Culex pipiens pallens strain to five other insecticides

目的了解淡色库蚊对化学杀虫剂的交互抗性,为合理选择杀虫剂提供依据。方法采用幼虫浸液法,测定经12代选育后的抗吡丙醚淡色库蚊幼虫对常用杀虫剂的半数致死浓度(LC_(50)),比较其对杀虫剂敏感性的变化。结果经过12代的抗性选育,淡色库蚊抗吡丙醚品系的抗性倍数达5.69倍,对溴氰菊酯、醚菊酯呈现低交互抗性,对双硫磷、倍硫磷以及除虫脲的交互抗性不明显。结论长期使用吡丙醚会产生抗性,并且可能会对其他杀虫剂产生交互抗性,应用时需注意使用频次以及杀虫剂种类的选择。

Objective To determine the cross-resistance spectrum of pyriproxyfen-resistant Culex pipiens pallens to other common insecticides and provide a basis for the optimal application of insecticides. Methods The larvae dipping method recommend by WHO was used to determine the median lethal concentration （LC^50）against insecticides tested. Results The resistance-ratio of Cx. pipiens pallens to pyriproxyfen increased to 5.69 after 12 generations of selection. There was a low cross resistance to deltamethrin and etofenprox, hut no cross resistance to temephos, baytex and diflubenzuron. Conclusion The resistance to pyriproxyfen and cross-resistance to other insecticides are expected to occur after long-time application of pyriproxyfen, therefore strategies must be implemented to mitigate the development of resistance and cross resistance.