基于正交实验的可溶性壳聚糖对黄瓜幼苗生长的影响

Effect of Soluble Chitosan on the Growth of Cucumber Seedling Based on Orthogonal Design Method

以黄瓜品种“鲁黄瓜三号”为试材，采用正交实验设计方法，研究了不同浓度的壳聚糖溶液以浸种和喷叶的不同处理方式对黄瓜幼苗生长的影响。结果表明：不同壳聚糖浓度处理均不同程度地促进了幼苗根系形态建成，增大了根系吸收面积，增强根系吸收养分的能力；同时提高了幼苗叶片中可溶性糖、可溶性蛋白质、叶绿素含量，提升了幼苗品质，最终提高穴盘幼苗质量。在试验范围内，0．3mg／mE浸种＋1．0mg／mL。喷叶处理（氏B3）最好，其壮苗指数最高，黄瓜苗根系长度及根系总体积也最大。0．3mg／mL浸种＋0．5mg／mL喷叶处理（气B2）次之，幼苗的株高、茎粗、根系总表面积最大，可溶性蛋白质含量和可溶性糖的含量也最高；综合各因素，该试验中培育黄瓜壮苗的浸种最适浓度为0．3mg／mL，喷叶适宜浓度在0．5～1．0mg／mL。

Taking＇Lu cucumber No. 3＇ cucumber variety as material, the effect of soluble chitosan in different methods of seed soaking and leaf spraying on cucumber seedling＇s growth were studied by the method of orthogonal experiment design. The results showed that different concentrations of soluble chitosan, could promote root coordinational growth, increase the root absorbing area of cucumber seeding, enhance nutrients absorption ability in the meantime, it enhenced chlorophyll content, soluble protein content, soluble sugar content, promoted the growth of the seeding, and finally improved the quality of cucumber seeding. Contrast with the control, A4 133 （0. 3 mg/mL seed soaking＋ 1.0 mg/mL leaf spraying） was the best in plant height,stem diamete,the total volume of roots. A4B2 was the second（0. 3 mg/mL seed soaking＋0. 5 mg/mL leaf spraying）, seedling plant height, stern diameter, total root surface area were the best, the contents of soluble protein and soluble sugar were the highest. In conclusion, the most proper concentration was seed soaking 0. 3 mg/mL,leaf spraying between 0. 5-1.0 mg/mL.