茶多酚与海藻酸钠膜对鲜切雏菊保鲜的影响

Effect of Tea Polyphenol Incorporated into Sodium Alginate-based Edible Coating on Preservation of Fresh-cut Bellis perennis

以鲜切雏菊为试材,采用涂膜处理的方法,研究茶多酚与海藻酸钠膜对鲜切雏菊鲜重变化、枯萎率、可溶性糖含量、可溶性蛋白质含量、多酚氧化酶(PPO)活性、过氧化物酶(POD)活性、丙二醛(MDA)含量、相对电导率的影响。结果表明:茶多酚与海藻酸钠膜对鲜切雏菊均有较好的保鲜效果,特别是2.0%茶多酚与0.05g/mL海藻酸钠结合处理鲜切雏菊效果最好。2.0%茶多酚+0.05g/mL海藻酸钠组能维持鲜切雏菊鲜重,在瓶插24d后,其鲜重变化率仅为-0.33%,能有效降低枯萎率,其中,当瓶插为24d时,只有16.56%的鲜切雏菊发生枯萎,而CK组的鲜切雏菊接近全部枯萎,0.05g/mL海藻酸钠组、1.0%茶多酚+0.05g/mL海藻酸钠组超过半数的鲜切雏菊已经发生枯萎;能减缓鲜切雏菊可溶性糖含量和可溶性蛋白质含量降低的速度;能延缓鲜切雏菊PPO活性高峰和POD活性高峰的出现,其PPO活性高峰和POD活性高峰出现时间都分别较CK组、0.05g/mL海藻酸钠组、1.0%茶多酚+0.05g/mL海藻酸钠组延迟了8、4、4d;MDA含量较其它3组上升得缓慢,而且在24d内MDA含量较小,特别在瓶插后期MDA含量明显小于CK组;相对电导率始终维持在较低水平,而其它3组的鲜切雏菊相对电导率则有大幅度地提高。

With the fresh-cut BeUis perennis as material,the fresh weight changes,withering rate, content of soluble sugar, content of soluble protein, polyphenol oxidase （PPO） activity, peroxidase （POD） activity, malonaldehyde （MDA） content and relative conductivity of fresh-cut Bellis perennis by tea polyphenol incorporated into sodium alginate-based edible coating using the method of coating processing were determined at this study. It showed that,tea polyphenol incorporated into sodium alginatebased edible coating had good effect on preservation of fresh-cut Bellis perennis, especially 0. 05 g/mL sodium alginate coating contained 2. 0% （w/v） of tea polyphenol. Due to the 0. 05 g/mL sodium alginate coating contained 2. 0% （w/v） of tea polyphenol,the fresh weight changes maintained well andthe rate of fresh weight changes only reached -0. 33% after 24 days;withering rate decreased perfectly, CK group of fresh-cut Bellis perennis faded nearly all and the group of 0. 05 g/mL sodium alginate and 0. 05 g/mL sodium alginate coating contained 1.0% （w/v） of tea polyphenol faded more than half, but only 16. 56% fresh-cut BeUis perennis become faded; the content of soluble sugar and content of soluble protein increased slowly; the maximum value of POD activity and PPO activity appeared slower,and there was a delay for 8 days,4 days,4 days separately compared with CK group,0. 05 g/mL sodium alginate group and 0. 05 g/mL sodium alginate coating contained 1.0% （w/v） of tea polyphenol group;MDA content of fresh-cut Bellis perennis increased slowly within 24 days, and MDA content was obviously smaller than that of CK group in the late;the relative conductivity stay at a lower lever, but the relative conductivity of other three groups of fresh-cut Bellis perennis had greatly improved.