不同蜜环菌菌株对木质纤维素利用率研究

Research on Utilization Ratio of Lignocellulose of Different ArmillariaStrains

以蜜环菌菌株为试材,通过测定2个生长时期8株蜜环菌木质纤维素利用率、生长速度、胞外酶活性,分析三者之间的相关性,揭示不同蜜环菌降解利用基质的差异和规律。结果表明:培养20d时,菌株KY4的木质纤维素利用率都高于其它菌株;培养40d时,菌株KY5的木质纤维素总利用率最高;2个生长期生长速度最快的都是菌株DJ1;通过差值分析,40d时纤维素酶活性大于20d,20d时漆酶活性大于40d,半纤维素酶活性变化无规律;A.mellea、A.cepistipes纤维素利用率与纤维素酶活性呈正相关,A.gallica纤维素利用率与纤维素酶活性呈负相关。

Taking Armillaria mellea as test material, armillaria lignocellulosic utilization rate, growth rate, extracellular enzyme activity at two growth period of 8 strains, and the correlation among the three were analyzed, and the difference of different Armillaria degradation using matrix and rules were studied. The results showed that,cultivation of 20 days, utilization of lignocellulose of strain KY4 was higher than other strains;cultivate 40 days, total utilization of lignocellulose of strais KY5 was the highest ;the fastest growth was strains of DJ1 during two growing periods;through the difference analysis,40 days of cellulase activity more than 20 days,the changes of activity of hemicellulase was bigger also had small change;A, mellea,A, cepistipes cellulose utilization and cellulase activity were positively correlated,A, gallica cellulose utilization was negatively correlated with cellulase activity.