电针治疗对酒精中毒性周围神经病大鼠压痛阈与坐骨神经传导速度的影响

Effects of Electroacupuncture on Alcoholic Peripheral Neuropathy Rats Pressure Pain Threshold and Conduction Velocity of Sciatic Nerve

目的该研究采用酒精自由饮的方法,进行CAPN的造模,通过酶联免疫及压痛阈的变化等观察,来探讨电针对慢性酒精中毒性周围神经病的麻木症状的作用机制,为针灸治疗慢性酒精中毒性周围神经病的临床推广提供新的理论依据。方法造模后的成年雄性WISTAR大鼠50只随机分成5组,模型组、电针组、弥可保组1、普通针刺组以及空白对照组各10只。弥可保组注射于下肢肌肉,左右腿交替,共治疗8周;电针组采用脾俞（双侧）、肾俞（双侧）、后三里（双侧）、环跳（双侧）、三阴交（双侧）、太溪穴（双侧）,进针后接KWD-808II脉冲电疗仪,采用疏密波,电流量以大鼠肢体肌肉轻度收缩为度,留针20分钟,隔日1次,共治疗8周;普针组取穴同上,不接电麻仪;空白对照组和模型组每日肌肉注射等量的生理盐水。压痛仪检测治疗前后的压痛阈,酶联免疫法检测大鼠背根神经节及坐骨神经组织中NGF的表达。结果酒精中毒损伤后坐骨神经传导速度降低,治疗2周达到一个小的高峰;2~4周坐骨神经传导速度出现一轻度回落趋势,但4周后又继续增加,始终处于高水平。结论电针可升高酒精中毒性大鼠坐骨神经传导速度以及降低大鼠四肢压痛阈值。

Objective This study adopted the method of alcohol free drink, CAPN model, through the observation of ELISA and pressure pain threshold changes, to explore the mechanism of electroaeupuneture treatment of numbness symptoms of chronic alcoholism peripheral neuropathy of, toxic peripheral neuropathy clinical promotion provided new theoretical basis for acupuncture and moxibustion treatment of chronic alcohol. Methods After the model of the 50 WISTAR adult male rats were randomly divided into 5 groups： model group, electro acupuncture group, Methycobal group 1, normal acupuncture group and control group, with 10 rats in each group. Methyeobal injection on lower limb muscles, legs around the turn, the course of the treatment was 8 weeks. Electroaeupuncture group used Pishu （bilateral） , Shenshu （bilateral） , Housanli （bilateral） , Huantiao （bilateral） , Sanyinjiao （bilateral） , Taixi （bilateral） , needle connected to the KWD-8081I pulse electrotherapy instrument, using density wave, the amount of current to the limb muscles of rats mild contraction for the degree, the needle stayed for 20 minute, 1 every other day, the course of the treatment was 8 weeks. The ordinary needle group selected the same acupoints as the Electroacupuncture group, and didn＇t connect with the electric acupuncture apparatus. The control group and model group were injected with equal amount of saline every day. Pressure pain instrument detected the threshold pressure pain before and after the treatment, enzyme linked immunosorbent assay for detection of the expression of rat dorsal root ganglion and the sciatic nerve tissue NGF. Results After injury of alcohol poisoning the conduction velocity of sciatic nerve dropped and 2 weeks of treatment up to a small peak; from 2 to 4 week, the conduction velocity of sciatic nerve emerged mild downward trend, while 4 week later the conduction velocity continued to increase. Remain at high levels. Conclusion d lowered limbs pain threshold in rats.