摘要
目的建立HPLC法测定参黄降浊胶囊中大黄素的含量。方法色谱柱为Acclaim 120 C18（4.6×250 mm,5μm）;流动相为甲醇∶水（75∶25）;流速1.0 ml·min-1;检测波长为219 nm。结果大黄素在0.5025~60.30μg·ml-1浓度范围内线性关系良好,r=0.9999,平均回收率为99.2%,RSD为0.4%（n=6）。结论此法简单可行,准确可靠,可用于该药品的质量控制。
Objective to establish a HPLC method for determination of emodin in yellow material capsule refs. Methods 120 C18 chromatographic column for Acclaim（4.6 x 250 mm, 5 microns）; Mobile phase of methanol： water（75：25）; The flow rate 1.0 ml, min-1; Detection wavelength of 219 nm. Results emodin in 0.5025 ~ 60.30 mu g·ml- 1 concentration range inside sex good, r = 0.9999,the average recovery was 99.2%, RSD was 0.4%（n = 6）. Conclusion this method is simple and feasible, accurate and reliable,can be used for the quality control of drugs.
出处
《中国中医药现代远程教育》
2015年第8期141-142,共2页
Chinese Medicine Modern Distance Education of China
