摘要
为了解新疆天山马鹿戊型肝炎病毒(HEV)的感染情况,试验采用双抗原夹心酶联免疫吸附试验(ELISA)法对新疆地区3个集约化鹿场和1个散养鹿场的410份马鹿血清进行抗戊型肝炎病毒Ig G抗体检测,比较不同鹿群及不同年龄段马鹿HEV抗体阳性率的差异。结果表明:所检测的血清总的抗体阳性率为32.4%(133/410),4个鹿群的抗体阳性率依次为:32.5%(65/200)、46.0%(46/100)、21.0%(20/95)、13.3%(2/15),且差异极显著(P<0.01);3个年龄段鹿群的抗体阳性率依次为:9.3%(7/75)、43.8%(92/210)、27.2%(34/125),且差异极显著(P<0.01)。说明新疆天山马鹿存在HEV的自然感染。
To understand the status of hepatitis E virus （HEV） infection in Tianshan wapiti （Cerus elophus songaricus） from Xinjiang, 410 copies of wapiti serum from three intensive deer farms and one free - range deer farm in Xinjiang were used for the detection of IgG antibodies against HEV using a double - antigen sandwich enzyme - linked immunoserbent assay, and the differences in the HEV antibody positive rates between different wapiti flocks and different age groups were compared. The result showed that the overall antibody positive rate of all detected serum was 32.4% （133/410） ; the antibody positive rates of four wapiti flocks were as follows： 32.5% （65/200）, 46% （46/100）, 21.1% （20/95） and 13.3% （2/15）, and there were extremely significant differences （ P 〈0. 01） among the four flocks; the antibody positive rates of the three age groups in the flocks were as follows ： 9.3% （7/75）, 43.8% （92/210）, and 27.2% （34/125）, and there were extremely signifi- cant differences （P〈0.01 ） among the three age groups. The results indicate that HEV natural infection exists in Tianshan wapiti from Xin-jiang.
基金
国家自然科学基金项目(31160513)
