不同双黄膏剂体外扩散的实验研究

Experimental Studies of Different Shanghuang Ointment on Transdermal Diffusion in Vitro

目的:研究3种双黄膏剂中黄芩苷在蛇蜕、小鼠腹皮的经皮扩散情况并对结果进行分析比较。方法:使用智能透皮试验仪对双黄冰凝胶(A)、双黄冰乳膏(B)、双黄乳膏(C)3种膏剂进行体外经皮扩散实验,测定黄芩苷的累积透过量和经皮扩散速率。结果:小鼠腹皮的累积透过量及经皮扩散速率均明显高于蛇蜕(P<0.01)。对于蛇蜕,各时间点A的累积透过量及经皮扩散率高于B、C(P<0.01),而B与C相比差异无统计学意义;对于小鼠腹皮,3种软膏累积透过量和经皮扩散率均无统计学意义。结论:采用不同皮肤会造成实验结果的不同。对于可增加角质层水合作用的凝胶剂,蛇蜕灵敏度高,干扰因素少,结果稳定。

Objective：To study the transdermal diffusion of baicalin in three Shanghuang Ointment and compare the effect of dif -ferent skin and ointment bases on diffusion .Methods：Intelligent transdermal test instrument was used to determine the cumulative permeating amounts of baicalin from Shanghuang Carbomer gel （ A） , Shanghuang Cream with borneol （ B） and without borneol （ C） . Then diffusion rate could be calculated .Results：the cumulative permeating amounts and transdermal diffusion rate on mice abdo-men skin were significantly higher than those on snake slough （ P〈0.01 ） .On snake slough , the cumulative permeating amounts and transdermal diffusion rate of baicalin in cream A was significantly higher than cream B and C （ P〈0.01 ,0.01 ） .The difference of the cumulative permeating amounts between two creams was not significant on mice skin .Conclusion：Different skin will make the different outcome .Shanghuang Carbomer gel can soften cuticles , and snake slough is superior to mice skin because of its high sensitivity, less interferences and excellent stability .