肌醇与D-手性肌醇抗氧化作用的研究

Study on antioxidant activity of inositol and D-chiro-inositol

目的:肌醇、D-手型肌醇对D-半乳糖致衰老小鼠体内抗氧化能力的研究。方法:采用颈背部皮下注射D-半乳糖的方法建立衰老小鼠模型,然后分别以100、200、400mg/(kg·bw)剂量的肌醇、D-手型肌醇灌胃。给药八周后,将小鼠处死,观察其对血清、肝脏和脑组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活性及丙二醛(MDA)含量和总抗氧化能力(T-AOC)的影响。结果表明:与模型组相比肌醇、D-手性肌醇各剂量组均能不同程度增加血清和组织中SOD和CAT的活性,提高T-AOC,降低MDA含量。说明肌醇和D-手型肌醇可以通过提高抗氧化酶的活性,显著提高D-半乳糖致衰老小鼠的抗氧化能力。

Objective ： Study on antioxidant activity of inositol and D-chiro-inositol.The aging mice model was established by subcutaneous injection of D-galactose at the neck. Six groups of mice were administered with inositol and DCI at doses of 100, 200, 400mg/kg for eight weeks. The antioxidant status in the aging mice was measured by determining the activities of superoxide dismutase （SOD）, catalase （CAT）, total anti-oxidant capability （T-AOC） and malondialdehyde （MDA） content in the serum, liver and brain. The results as follows ： compared with control group inositol, D- chiro-inositol significantly enhanced activities of SOD, CAT T-AOC, and decreased MDA content in the serum, liver and brain. It indicated that inositol and DCI could improve the antioxidant enzymes activity and signifcantly increase the antioxidant ability of mice, especially in the high dosage group.