响应面优化蛹虫草多糖脱蛋白工艺研究

Optimization of deproteinization for polysaccharide from cordyceps by response surface analysis

以人工培养的蛹虫草(Cordyceps militaris L.)为原料,以多糖损失率和蛋白去除率为指标,采用木瓜蛋白酶对蛹虫草粗多糖进行脱蛋白处理,通过单因素与响应面分析法探索蛹虫草多糖除蛋白的优化工艺条件。结果表明:当酶液与样液的体积比为1.72∶1、酶解温度为64℃、p H 6.23、酶解时间为3 h时,蛋白质脱除率为68.9%,多糖损失率为10.13%,结合一次Sevage法后,蛋白脱除率与多糖损失率分别为83.37%、19.43%。

In the study, cultured Cordyceps militaris L. was used as raw materials, taking polysaccharide losing rate and protein removal rate as indexes, papain was used to deprotein from cordyceps polysaccharide, and the optimal conditions were determined by single factor experiment and response surface analysis. The results showed that, the optimal conditions were as follows： volume ratio of enzyme and polysaccharides solution was 1.72, temperature was 64~C, pH was 6.23, for 3 h. Under these conditions, protein removal rate was 68.9%, and polysacchafide losing rate was 10.13%. After Sevage method once time, protein removal rate and polysaccharide losing rate were 83.37% and 19.43%, respectively.