不同剂量对乙酰氨基酚诱导大鼠急性肝损伤的研究

Research on Different Doses of Acetaminophen Inducing Acute Liver Injury in Rats

通过测定SD大鼠血浆及肝组织中六种酶的活性或含量,探索对乙酰氨基酚短期内对大鼠肝脏的损伤作用。SD大鼠实验组分别皮下注射75、150、250 mg·kg-1剂量的对乙酰氨基酚,对照组注射生理盐水且于给药后1、6、24 h分别采取血浆和肝组织,采用比色法检测各项指标。结果显示:高剂量组大鼠血浆中AST(356.72±43.87 U·L-1)和ALT(325±40.56 U·L-1)以及肝匀浆中GSH(4.55±0.53 mmol·prot-1)、SOD(90.1±12.23 U·g-1)和MDA(230.24±40.43 nmol·g-1)与对照组相比,差异极显著(P<0.01),说明250 mg·kg-1剂量的对乙酰氨基酚可对大鼠肝脏产生急性损伤作用。

In order to illustrate the acetaminophen role on rat liver damage,the activity or levels of 6 kinds of enzyme were detected in rat plasma and liver tissue. SD rats were injected subcutaneously 75,150,250 mg·kg^-1 dose of acetaminophen in experimental group,and injected with administered saline after 1,6 and 24 h in control group,and plasma and liver tissue were used colorimetric detection indicators. The high group of rat plasma AST（356.72±43.87 U·L^-1）and ALT（325±40.56 U·L^-1）and in liver homogenates of GSH（4.55±0.53 mmol·prot^-1）,SOD（90.1±12.23 U·g^-1）and MDA（230.24±40.43 nmol·g^-1）compared with the control group,and the difference was significant（P〈0.01）.In the experiment,250 mg·kg^-1 dose of acetaminophen could damage the liver in rats.