耐喹诺酮类药物铜绿假单胞菌的质粒基因研究

Study of plasmid-mediated quinolones antibacterial in pseudomonas aeruginosa

目的研究耐喹诺酮类药物铜绿假单胞菌(PA)的质粒基因,并分析其耐药机制。方法临床分离的耐喹诺酮类药物的PA 100株,采用PCR法检测质粒介导的喹诺酮类耐药基因qnrA、qnrB、qnrC、qnrD、qnrS、qepA、aac(6′)-Ib-cr,并对阳性结果进行测序分析。结果 100株PA中检测出含有qnrA基因的菌株34株(34%);qnrB基因79株(79%);qnrD基因14株(14%);qnrS基因8株(8%);aac(6′)-Ib-cr基因55株(55%);未检测出qnrC和qepA基因的菌株。结论质粒携带的qnrA、qnrB/aac(6′)-Ib-cr耐药基因是PA耐喹诺酮类药物的主要机制。

Objective To study the quinolones resistance plasmid genes in pseudomonas aeruginosa and ana- lyze its mechanism. Methods pseudomonas aeruginosa samples were collected for the detection of qnrA,qnrB,qnrC, qnrD,qnrS,qepA and aac（6＇）-Ib-cr gene using PCR, then the positive samples were detected by sequence analysis. Results qnrC and qepA gene were not detected in strains resistance to Ciprofloxacin. the positive rate of qnrA,qnrB, qnrD,qnrS,aac（6＇）-Ib cr gene is 34%,79% ,14%,8%,55% respectively. Conclusion plasmid with qnrA,qnrB,aac （6＇）-Ib-cr gene is the main mechanism in pseudomonas aeruginosa to quinolones antibacterial.