衣壳蛋白和甲基转移酶对戊型肝炎病毒的基因分型

Capsid protein and methyltransferase can be used for genotyping of hepatitis E virus

目的探讨人戊型肝炎病毒(HEV)的部分编码区序列能否作为其基因组分型的依据。方法从GenBank中查找并下载全基因组测序完整的能感染人类的HEV基因组及蛋白序列共35株,用Clustal W程序对35株HEV全序列以及部分编码区序列进行多序列比对,并用treev 32绘制基因进化树,研究其进化关系。结果HEV的基因组全长7.5kb;而衣壳蛋白和甲基转移酶的编码序列分别为1 982bp和545bp,这2种蛋白的多序列比对和进化分析结果与完整基因组的分型结果相符。结论衣壳蛋白和甲基转移酶可作为HEV的分型依据,且是一种更简便、快捷的方法。

Objective To understand the part of the coding region can be used as the basis for the genotyping of hepatitis E virus（HEV）. Methods Thirty-five full-length sequences of HEV currently available in GenBank were download,and sequence alignment and evolutionary analysis of these thirty-five full-length sequences and part of the coding region were done. Results The complete genome of HEV was 7. 5 kb in length, while Capsid Protein was 1 982 bp, Methyltransferase was 545 bp. The result of sequence alignment and evolutionary analysis of the two kinds of protein were consistent with the complete genome of HEV. Conclusion The sequence of Capsid Protein and Meth- yltransferase is shorter,and can be used as the basis for the genotyping of HEV,which is more simple and rapid.