镰刀菌Fusariumsp sp.JN 158色素的鉴定及其抗肿瘤活性

Pigment Production by Fermentation of Fusarium(Fusarium sp.JN158) and It's Anti-Cancer Activity

通过对镰刀菌发酵所产色素的分离及结构的分析,筛选其抗肿瘤的活性成分。采用形态与分子生物学方法对镰刀菌株鉴定,利用HPLC的对该菌株发酵所产的色素进行分离,分离的产物用紫外、红外、核磁进行结构鉴定,使用MTT的方法筛选抗肿瘤的活性成分。结果表明:该镰刀菌所产色素共6个不同的峰,相对分子质量均在380左右,其中VI号峰结构新颖,为花青苷类色素,分子式为C17H18O10,能抑制A539、MCF-7、MKN-45、Hep G2、SW620五种不同肿瘤细胞的增殖,并呈现浓度的依赖性,其中MCF-7乳腺癌细胞抑制最明显(P<0.01),故VI号峰色素可能是一种很有前景的防治乳腺癌的化合物。

Through the separation and structural analysis of the pigment produced by Fusarium fermentation, the research aims to screen the anti-tumor active components. Fusarium was identified by using morphological and molecular biology methods. The pigmen was isolated by HPLC,the production structure was identified by UV, IR,NMR., and the screening of anti-tumor activity was by MTT. The results showed that the pigment from Fusarium produced a total of six different peaks, the molecular weight is about 380,the VI peak,which is the anthocyanins,can inhibit the A539,MCF-7,MKN-45,HepG2,SW620 five different tumor cell proliferation in a concentration- dependent manner, among them MCF-7 breast cancer cells were inhibited most （P〈0.01）, so the VI compound with a new structure from Fusarium sp JN 158,may be a good treatment for breast cancer.