枯草芽孢杆菌产过氧化氢酶的优化与工业化

Optimization and Industrial Production of Catalase in Bacillus subtilis

为了实现过氧化氢酶的工业化生产,作者对实验室前期构造成功的B.subtilis WSHDZ-01(p STOP1622-kat A)进行了3 L发酵罐的发酵验证,并且通过数据分析优化了碳氮源浓度,使得过氧化氢酶酶活达到35 398 U/m L,比优化前提高53.41%。在此基础上,分别进行了24 L发酵罐和500 L发酵罐的放大实验,最高酶活分别达到28 940、23 190 U/m L。结果表明,该过氧化氢酶在产酶水平上已经达到工业化生产需求,为更大规模的工业化生产奠定坚实的基础。

Microorganism catalase, which is an important industrial enzyme, plays an important role in textile industry. To realize the industrial production of catalase,the fermentation on B. subtilis WSHDZ-01 （pSTOP1622-katA） ,which was constructed before,was conducted in 3 L fermentor. Furthermore,the enzyme activity reached 35 398 U/mL after optimization of carbon and nitrogen concentration,which was increased by 53,41% compared with that before optimization. Based on this result,the fermentations in 24 L and 500 L fermentor were carried out,respectively and the maximum enzyme activity was 28 940 U/mL and 23 190 U/mL respectively. The result showed the yield of catalase has met the requirement of industrial production, which lay a solid foundation for larger-scale industrial production.