摘要
从风干香肠中分离得到一株生长良好的乳酸菌ORC4,菌株能产乳酸、革兰氏染色阳性、接触酶阴性。经系统发育分析,结合菌落形态、细胞形态、生化反应试验,确定菌株ORC4为绿色魏斯氏菌。绿色魏斯氏菌ORC4的完整和破碎细胞对O2-·、DPPH·和·OH的清除率都与细胞浓度呈正相关。在细胞浓度为5×108CFU/m L时,菌株ORC4破碎细胞对O2-·、DPPH·和·OH的清除率都高于完整细胞,分别为45.17%、56.17%和54.01%。菌株ORC4还能清除培养基中最多为35.87μg/m L的胆固醇,单位细胞干重的胆固醇清除率为19.89μg/mg。
A Gram (+),non-motile,facultatively anaerobic,rod-or coccoid-shaped bacterium, designated strain ORC4,was isolated from the dry fermented sausages. The 16S rRNA gene sequence analysis showed that strain ORC4 belonged to the genus 1Veissella,with highest sequence similarity to 1Veissella viridescens NRIC1536 (100%). The intact cells and intracellular extract of strain ORC4 could inhibit 02- ,DPPH ,and -OH. Three free radicals-scavenging ratios of intracellular extract were positively correlated with bacterial density,respectively,the intact cells could do the same. The intracellular extract had higher scavenging ratios on three free radicals than the intact cells. At a concentration of 5 - 108 CFU/mL, scavenging ratios of intracellular extract on O2+ ,DPPH. and -OH were 45.17% ,56.17% and 54.01% respectively. In addition,strain ORC4 could reduce 35.87 la g/mL cholesterol,and the unit cell dry weight of cholesterol removal rate was 19.89 μg/rag.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2015年第5期512-516,共5页
Journal of Food Science and Biotechnology
基金
国家自然科学基金项目(31070005)
中央高校基本科研业务费专项资金资助项目(DC12010103
DC120101034)
关键词
风干香肠
绿色魏斯氏菌
16S
r
DNA
系统发育树
抗氧化作用
降胆固醇作用
dry fermented sausages, Weissella viridescens, 16S rDNA,phylogenetic tree,antioxidative effect, cholesterol-reducing ability