三角帆蚌糖蛋白的盐溶液浸提及抗氧化活性

Salty Extraction and Antioxidant Activities of Glycoprotein from Hyriopsis cumingii

以三角帆蚌脏器为试验材料,以Na Cl溶液为提取溶剂,研究三角帆蚌脏器糖蛋白的低温提取、分离及抗氧化活性。通过单因素试验和正交试验,确定三角帆蚌脏器糖蛋白最优提取条件为:提取温度4℃、Na Cl溶液浓度0.3 mol/L、料液比1∶15、提取时间9 h。在此条件下,糖蛋白得率(以糖含量计)为(9.259±0.083)%,糖蛋白得率(以蛋白质含量计)为(3.763±0.107)%。提取液经硫酸铵分级沉淀得三个组分糖蛋白粗品。体外抗氧化实验表明,三个组分都具有清除超氧阴离子自由基和羟基自由基的能力,但清除能力不同。10%SDS-PAGE电泳后经考马斯亮蓝R250和PAS染色,表明3个组分含有不同的糖蛋白谱带。

Hyriopsis cumingii visceral organ and Nacl solution were used as material and extraction liquor to study the hyriopsis cumingii glycoprotein extraction and separation under low temperature and the antioxidant activity. Through single factor experiment and orthogonal experiment,the optimized extraction condition was as below：extraction temperature at 4℃ ,0.3 mol/L NaC1 solution,solid-liquid ratio in 1 ： 15 ,and extraction for 9 h. Glycoprotein extraction rates under optimal condition interms of sugar content and protein content were （9259 ± 0.083）% and （3.763 ± 0.107）% respectively. Crude glycoprotein sample,that comprises three different components, was obtained ,after the extraction liquor was fractionally precipitated by ammonium sulfate. The different component had a different ability to scavenge surperoxide anion radicals and hydroxyl radicals. Crude glycoprotein sample was Electrophoresed by 10% SDS-PAGE and dyed by Coomassie Brilliant Blue R250 and PAS,and the result showed the three components contained different glycoprotein bands.