光合细菌与纳豆菌的混合培养及混合处理养殖水的研究

Study on the Mixed Culture of Photosynthetic Bacteria with Bacillus Natto and its Effects on Aquaculture Water

采用正交试验优化纳豆菌和光合细菌混合培养时的接种量及菌剂的装液量,使光合细菌活菌数在最短的时间内达到最大值。结果显示:光合细菌接种量20%、纳豆菌接种量2%、每250 mL三角瓶菌剂装液量60%培养效果最好。同时研究了光合细菌与纳豆菌混合菌剂对养殖水的亚硝酸盐氮以及氨氮的降解能力,发现2种菌的最佳混合比例为1∶1(v∶v),混合菌菌剂添加量与亚硝酸盐的降解作用成正相关;由于纳豆菌能将培养基中的有机氮转化为氨氮,混合菌剂对养殖水氨氮的降解效果随着添加量的增加而减弱。

In order to make photosynthetic bacteria reached the maximum number of viable cells in the shortest possible time,,the culture inoculation and liquid volume of agents for the mixed culture of natto and photosynthetic bacteria were studied.with orthogonal test.The results showed that 20% of photosynthetic bacteria inoculation,natto inoculum 2%,agent liquid volume 60%in 250 mL flask culture was the best.The effects of mixture culture of photosynthetic bacteria and natto bacteria on the degradation of nitrite nitrogen and ammonia nitrogen in aquaculture water were also studied.The results showed that the best effect was received when two kinds of bacteria was mixed at 1∶1（v∶v）,and the amount of mixed bacterial agents was positive correlation with the degradation of nitrite.Because natto bacteria could transfer organic nitrogen of medium into ammonia,so the increasing of the added amount of mixed inoculant weakened the degradation effects of ammonia nitrogen in aquaculture water.