枯草芽孢杆菌CS16发酵条件及其抑菌活性物质研究

Fermentation Conditions and Antagonistic Substances of Bacillus subtilis CS16

对香蕉枯萎病病原菌有较强抑制作用的枯草芽孢杆菌CS16进行发酵条件优化,通过单因素和正交试验得到CS16抑菌活性较强的培养基配方和发酵条件。结果显示:培养基配方为玉米粉0.5%,豆粕1.5%,K2HPO40.01%;发酵条件为pH 7.5,接种量4%,装瓶量50mL/250mL,培养时间24h,发酵温度30℃时,菌量达3.3×109cfu·mL-1,与优化前相比提高了42倍,抑菌活性提高了7%。抑菌活性物质测定结果表明CS16中抑菌活性物质可分为杀死病原菌和抑制病原菌菌丝生长2类物质。

Bacillus subtilis CS16 could effectively inhibit banana fusarium wilt pathogen.The formation conditions for CS 16 were optimized in this study.By single factor orthogonal experiment,the fermentation medium and culture conditions were selected to improve the antibacterial activity of CS 16.The results showed that the optimal culture conditions were fermentation medium consisting of corn flour 0.5%,soybean meal 1.5%,K2HPO40.1%,with culture pH 7.5,inoculation valum 4%,culture fluid volume 50mL/250 mL.In which,by culturing 24hat30℃,the biomass of CS 16 reached to 3.3 × 109 cfu· mL-1 that was 42 times higher than that of control.Meanwhile,the antibacterial activity of CS 16 increased by 7%.It was found that antimicrobial compounds of CS 16 could be grouped into two types：to kill the pathogenic cells or to inhibit the growth of pathogenic hyphae.