摘要
研究胃蛋白酶对红豆蛋白的水解工艺.考察酶浓度、pH、温度、水解时间等因素对红豆蛋白水解度的影响.结果表明:制备的胃蛋白酶能够较好地水解红豆蛋白,其最佳水解工艺为:温度40℃、酶浓度7U/g干蛋白、pH1.5、水解时间4h,其最佳水解度为30.97%.
Red bean protein is hydrolyzed by pepsin from stomach of swine. The effects of enzyme concentration, temperature, pH and time on enzymatic hydrolysis are discussed. The results show that the optimal conditions for producing peptides from red bean protein by pepsin are enzyme concentration of 7 U/g dry protein, pH of 1.5, the temperature of 40℃ and hydrolysis time 4.0 h. The degree of hydrolysis is 30.97%.
出处
《湖南工程学院学报(自然科学版)》
2015年第2期73-76,共4页
Journal of Hunan Institute of Engineering(Natural Science Edition)
基金
湖南省教育厅科研资助项目(11C0329)
国家大学生创新创业训练计划项目(201411342004)
湖南省大学生研究性学习和创新性实验计划项目(湘教通[2014]248号)
关键词
胃蛋白酶
红豆蛋白
水解度
pepsin
red bean protein
degree of hydrolysis