Sysmex CA7000血凝仪2种方法测定纤维蛋白原结果的比较

Comparison of two methods detecting fibrinogen on Sysmex CA7000

目的：探讨 Sysmex CA7000血凝仪2种方法测定纤维蛋白原（Fib）结果差异及其相关性。方法使用 Sysmex CA7000型全自动血凝分析仪及其原装配套试剂，分别用凝血酶原时间（PT ）衍算（PT‐Fib）法及凝血酶法（Clauss 法）同时测定临床常规血凝标本 Fib 水平。回顾性随机抽取 PT‐Fib 法检测的 Fib 水平在1．00～1．99、2．00～2．99、3．00～3．99、4．00～4．99 g ／L范围内的各21例患者数据，分析其与 Clauss 法测定结果差异及其相关性。结果 PT‐Fib 法检测 Fib 水平在1．00～1．99、2．00～2．99、3．00～3．99、4．00～4．99 g／L 时，2种方法之间检测结果差异有统计学意义（P＜0．05），PT‐Fib 法结果明显低于 Clauss 法，4组浓度平均值分别低15．2％（－4．9％～38．3％）、27．2％（－0．9％～49．4％）、24．6％（－3．7％～35．6％）、32．7％（15．7％～48．2％），2种方法测定结果呈正相关。结论测定血浆 Fib 时，PT‐Fib 法与 Clauss 法有差异，建议临床实验室把 Clauss 法作为Fib 常规定量测定方法。

Objective To evaluate the accuracy of using prothrombin time （PT ） derivative algorithm（PT‐Fib） method to detect fibrinogen（Fib）content in plasma samples with Sysmex CA7000 automatic coagulometer and its correlation with results of Clauss method .Methods Clauss method and PT‐Fib method were used to detect Fib concentration in plasma of patients′ blood samples by Sysmex CA7000 automated blood coagulation analyzer and supporting reagent .Data were randomly collected as the Fib content were in 1 .00 - 1 .99 ,2 .00 - 2 .99 ,3 .00 - 3 .99 ,4 .00 - 4 .99 g/L detected by PT‐Fib method ,each range with 21 patients .The difference and correlation of two methods were analyzed .Results There was significant statistical difference between PT‐Fib meth‐od and Clauss method（P〈 0 .05） when the level of Fib detected by PT‐Fib method were 1 .00 - 1 .99 ,2 .00 - 2 .99 ,3 .00 - 3 .99 , 4 .00 - 4 .99 g/L （n= 21） .The result of the PT‐Fib method was obviously lower than those of the Clauss method on average 15 .2%（ - 4 .9% - 38 .3% ） ,27 .2% （ - 0 .9% - 49 .4% ） ,24 .6% （ - 3 .7% - 35 .6% ）and 32 .7% （15 .7% - 48 .2% ）respectively ,and results of the two methods were positively correlated .Conclusion Fib value calculated by the PT‐Fib method in the fully automatic coagu‐lation Sysmex CA7000 analyzer is obviously lower than that of the Clauss method .It is suggested that the Fib detection should be changed to adopt Clauss method while detecting Fib content in plasma samples with Sysmex CA7000 automatic coagulometer .