参苓白术散对胃癌IV期患者外周血CD4+CD25+Tregs的影响

Effects of Shenling Baizhu Powder on CD4+CD25+Tregs in Peripheral Blood of Patients with Stage IV Gastric Cancer

目的探讨参苓白术散对胃癌IV期患者外周血CD4+CD25+Tregs、Foxp3 m RNA及血清细胞因子IL-10、TGF-β1的影响。方法将40例胃癌IV期患者随机分为观察组、对照组各20例。对照组予对症支持治疗,观察组对症支持治疗同时予参苓白术散治疗,治疗4 w后观察两组患者外周血CD4+CD25+Tregs、单核细胞Foxp3 m RNA及血清IL-10、TGF-β1浓度的变化。结果治疗后观察组患者外周血CD4+CD25+Tregs、单核细胞Foxp3 m RNA及血清IL-10、TGF-β1浓度均显著降低(P<0.05),对照组治疗后各项指标无明显变化(P>0.05),两组治疗后比较差异有统计学意义(P<0.05)。结论参苓白术散能降低胃癌IV期患者外周血CD4+CD25+Tregs和单核细胞Foxp3 m RNA的表达,降低抑制性细胞因子IL-10、TGF-β1浓度,具有免疫调节作用。

Objective To examine the effect of Shenling Baizhu powder on the counts of CD4＋CD25＋ Regulatory T cells,and the expression of Foxp3 m RNA, and IL-10 and TGF-β1 in peripheral blood of patients with stage IV gastric cancer.Methods 40 patients with stage IV gastric cancer were randomly divided into the observation group and control group, 20 patients in each group. The patients in control group were treated by conventional therapy; while the therapeutic group was treated with Shenling Baizhu powder and conventional therapy. After 4 weeks of treatment, the counts of CD4 ＋CD25 ＋Regulatory T cells, the expression level of Foxp3 m RNA of monocytes, IL-10 and TGF-β1 in peripheral blood were observed.Results The counts of CD4 ＋CD25 ＋Regulatory T cells and the expression of Foxp3 m RNA of monocyte in peripheral blood,the serum concentration of IL-10 and TGF-β1 were all decreased（P 0.05）, The parameters in the control group had no obvious changes（P〈0.05） and there are significant differences between these two groups（P〈0.05）. Conclusion Shenling Baizhu powder showed immunomodulatory effect by reducing the counts of CD4 ＋CD25 ＋Regulatory T cells, the expression of Foxp3 m RNA in monocyte in peripheral blood, and the serum concentration of IL-10 and TGF-β1.