摘要
为研究MDCK细胞的生长及代谢动力学特征,试验采用低密度静置培养,每天测定细胞生长密度和活力,并利用多参数生化分析仪测定培养液中Gluc、Lac、Gln和NH4+的含量,计算细胞指数生长期的比代谢速率。结果表明:MDCK细胞生长曲线呈"S"型,最大增殖密度为53.8×104 upf·mL-1,倍增时间为24.2h;对数生长期Gluc和Gln的比代谢(消耗速率为-2.20 mg·(106cells·d)-1和-3.85μmol·(106cells·d)-1,Lac和NH4+的比生成)速率为2.25mg·(106cells·d)-1和2.22μmol·(106cells·d)-1。
In order to research growth and metabolism dynamics characteristics of MDCK cells,low cell density cultured method was used to determine cell density and viability every day. The content of Gluc, Lac,Gln and NH4^＋ in medium were determined by multi-parameter biochemical analyzer, and the specific metabolic rate were calculated. The results showed that the growth curve of MDCK cells was ＂S＂ type,the largest density was 53.8× 10^4 upf· mL^-1 and the doubling time was 24. 2 h. Specific consumption rate of Gluc and Gln were -2.20 mg. （10^6cells·d）^-1 and -3.85 μmol·（10^6cells·d）^-1 in logarithmic phase,and specific generation rate of Lac and NH4^＋ were 2.25 mg. （10^6cells·d）^-1 and 2.22 μmol·（10^6cells·d）^-1.
出处
《黑龙江农业科学》
2015年第6期71-74,共4页
Heilongjiang Agricultural Sciences
基金
国家民委领军人才计划资助项目
国家自然科学基金资助项目(31160033)
甘肃省科技计划资助项目(1308RJZA142)
西北民族大学中央高校基本科研业务费专项资金资助项目(31920150029
31920130066)
