丹参酮I和二氢丹参酮I对人胃癌细胞MGC-803、乳腺癌细胞MCF-7的抗肿瘤活性研究

The Study on the Anti-tumor Activity of Tanshinone I and Dihydrotan-shinone I on MCF-7 Human Breast and MGC-803 Gastric Cancer Cell Lines

目的:比较研究丹参酮I和二氢丹参酮I对人胃癌细胞MGC-803、乳腺癌细胞MCF-7的抗肿瘤活性。方法:从白花丹参中分离、纯化丹参酮I和二氢丹参酮I成分,采用MTT法测定对肿瘤细胞的生长抑制作用,同时采用流式细胞仪检测细胞周期的改变以及凋亡情况。结果:丹参酮I、二氢丹参酮I对MCF-7几乎没有生长抑制作用,对MGC-803有很明显的生长抑制作用,同时可明显阻滞人胃癌细胞MGC-803的细胞周期,使细胞核裂解呈现碎片状而产生凋亡小体,且其凋亡率成明显的上升趋势。结论:丹参酮I、二氢丹参酮I通过诱导细胞凋亡,对MGC-803肿瘤细胞具有很好的抑制作用,而对MCF-7几乎没有活性。

Objective： To investigate the anti-tumor activity of tanshinone I and dihydrotanshinone I on the MCF-7 human breast cancer cell line and MGC-803 Gastric cancer cell line. Methods： Tanshinone I and di- hydrotanshinone I were isolated from Salviamiltiorrhiza Bunge. The anti-tumor activity of the compounds was evaluated by MTT tests. The apoptosis case was observed during the whole experiment with the optimal dosing concentration by fowctometry technology. Results： There was no inhibited effect in MCF-7 human breast cancer cell line and MGC-803 Gastric cancer cell line treated with tanshinone I and dihydrotanshinone I. On the contrary, the two compounds can significantly suppressed the grow of MGC-803 cell line by leading to the cell cycle arrest in MGC-803 cell line, and the cleavage of the nucleus to pruduceapoptotic bodies. The apoptosis rates showed a significant upward trend. Conclusion： Tanshinone I anct dihydrotanshinone I can induced apoptosis to display notent anti-tumor activity in human MGC-803 Gastric cancer cell line.