摘要
通过对斑马鱼肌肉注射不同浓度毒死蜱以及维生素E,研究斑马鱼血清中过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活力变化情况以及维生素E的抗氧化作用。试验分为5组,依次为对照组和低、中、高浓度毒死蜱组(0.002、0.02、0.2 mg/kg)以及高浓度联合维生素E(0.2 mg/kg+VE)治疗组。结果表明,毒死蜱不同浓度组短时间(24 h)作用均使两种酶活性升高(P﹤0.01),加维生素E组活性增高更显著;长时间(168 h)作用则明显抑制酶活性(P﹤0.01),维生素E组酶活性变化较平缓。表明毒死蜱对斑马鱼机体抗氧化系统损伤作用明显,维生素E对CAT和SOD活力有保护作用。
The effects of muscle injection with different doses of chlorpyrifos （0.002, 0.02, 0.2 mg/kg） and one treatment group （chlorpyrifos＋vitamin E） on the activity of catalase （CAT） and superoxide dismutase （SOD） in serum of zebrafish were studied at different sampling times （0,24,48,96 ,168 h）. The results showed that chlorpyrifos with different concentration increased activity of the two enzymes at the time of 24 h, and decreased significantly at other time. The effects of treatment group on enzymes were less obvious than that of other groups. It is indicated that chlorpyrifos significantly affects the antioxidant of zebrafish and vitamin E protects CAT and SOD.
出处
《湖北农业科学》
2015年第7期1590-1593,共4页
Hubei Agricultural Sciences
基金
国家级大学生创新创业训练计划项目(201210927025)
湖北省教育厅科学研究计划项目(Q20122802
B2013050)
