摘要
为了筛选对黄瓜疫霉菌有抑制作用的生防菌,采用土壤稀释法从湖南、北京等地采集的土壤样品中分离到269株放线菌和细菌,以黄瓜疫霉菌为靶标菌,通过平板对峙培养法,筛选出2株对黄瓜疫霉菌有明显拮抗作用的生防菌株。采用5倍稀释发酵液对黄瓜疫霉菌进行抑菌活性检测,放线菌X54和细菌P3的抑制率达到70%以上。通过培养形态特征观察、生理生化试验及16Sr DNA分子鉴定,放线菌X54为弗吉尼亚链霉菌(Streptomyces virginiae),细菌P3为地衣芽孢杆菌(Bacillus licheniformis)。
In order to screen effective antagonistic agents to Phytophthora melonis, 269 strains were isolated from collected soil in Hunan and Beijing with gradient dilution method. 2 strains showed obvious antagonistic effects to Phytophthora melonis by using agar plate dual culture, and strain X54 and strain P3 were finally screened out. The diluted 5 times filtration showed their inhibition rates were over 70%. Through observation of culture morphological characteristics, physiological and biochemical experiment, and molecular identification of 16S rDNA, we found that actinomyeete X54 was Streptomyces virginiae and bacterial P3 was Bacillus licheniformis.
出处
《中国蔬菜》
北大核心
2015年第6期23-27,共5页
China Vegetables
基金
农业部公益行业计划项目(201003004)
湖南省高校创新平台开放基金项目(13K064)
关键词
黄瓜疫霉菌
生物防治
弗吉尼亚链霉菌
地衣芽孢杆菌
Phytophthora melonis
Biological control
Streptomyces virginiae
Bacillus licheniformis
