2型糖尿病大鼠肝脏胰岛素受体表达的变化和丝胶的作用

CHANGES OF INSULIN RECEPTOR IN LIVER OF TYPE 2 DIABETES MELLITUS RATS AND ACTIONS OF SERICIN

目的:研究2型糖尿病大鼠肝脏胰岛素受体(insulin receptor,IR)表达的变化及丝胶的保护作用。方法:36只SD大鼠随机分为正常对照组、糖尿病模型组和丝胶治疗组3组,每组12只大鼠。采用链脲佐菌素连续腹腔注射法制作2型糖尿病大鼠模型,待模型成功建立后,丝胶治疗组大鼠给予丝胶(2.4g/kg/d)灌胃35d。采用葡萄糖氧化酶法检测各组大鼠的空腹血糖,Western Blotting法和RT-PCR检测大鼠肝脏IR蛋白和m RNA的表达。结果:与糖尿病模型组大鼠比较,丝胶治疗组大鼠肝脏IR蛋白和m RNA的表达明显升高(P<0.01)。结论:丝胶可能通过上调2型糖尿病大鼠肝脏IR的表达在降血糖中发挥作用。

Objective：To study the changes of insulin receptor （IR） expression in liver of type 2 diabetes mellitus rats and protective actions of sericin.Methods：36 male SD rats were randomly divided into the following 3 groups （n=12）：normal control group, diabetes model group and sericin treatment group. The rats with type 2 diabetes mellitus were induced by continuous intraperitoneal injection of streptozotocin. After successfully establishing diabetes rats model, the rats in sericin treatment group were lavaged with sericin （2.4g/kg/d） for 35 days. Glucose oxidase was used to detect the fasting blood glucose; Western Blotting and RT-PCR were used to detect the IR protein and mRNA expression in the liver of rats.Results：Compared with rats in diabetes model group, the IR protein and mRNA expression in liver of rats in sericin treatment group increased obviously （P〈0.01）.Conclusions： One of the mechanisms of sericin decreasing blood glucose maybe secrin can upregulate IR expression in liver of type 2 diabetes mellitus rats.