摘要
目的:研究2型糖尿病大鼠肝脏胰岛素受体(insulin receptor,IR)表达的变化及丝胶的保护作用。方法:36只SD大鼠随机分为正常对照组、糖尿病模型组和丝胶治疗组3组,每组12只大鼠。采用链脲佐菌素连续腹腔注射法制作2型糖尿病大鼠模型,待模型成功建立后,丝胶治疗组大鼠给予丝胶(2.4g/kg/d)灌胃35d。采用葡萄糖氧化酶法检测各组大鼠的空腹血糖,Western Blotting法和RT-PCR检测大鼠肝脏IR蛋白和m RNA的表达。结果:与糖尿病模型组大鼠比较,丝胶治疗组大鼠肝脏IR蛋白和m RNA的表达明显升高(P<0.01)。结论:丝胶可能通过上调2型糖尿病大鼠肝脏IR的表达在降血糖中发挥作用。
Objective:To study the changes of insulin receptor (IR) expression in liver of type 2 diabetes mellitus rats and protective actions of sericin.Methods:36 male SD rats were randomly divided into the following 3 groups (n=12):normal control group, diabetes model group and sericin treatment group. The rats with type 2 diabetes mellitus were induced by continuous intraperitoneal injection of streptozotocin. After successfully establishing diabetes rats model, the rats in sericin treatment group were lavaged with sericin (2.4g/kg/d) for 35 days. Glucose oxidase was used to detect the fasting blood glucose; Western Blotting and RT-PCR were used to detect the IR protein and mRNA expression in the liver of rats.Results:Compared with rats in diabetes model group, the IR protein and mRNA expression in liver of rats in sericin treatment group increased obviously (P〈0.01).Conclusions: One of the mechanisms of sericin decreasing blood glucose maybe secrin can upregulate IR expression in liver of type 2 diabetes mellitus rats.
出处
《承德医学院学报》
2015年第3期188-190,共3页
Journal of Chengde Medical University
基金
国家自然科学基金项目(81441133)
河北省自然科学基金项目(H2013406096)
河北省高校重点发展学科"人体解剖与组织胚胎学"建设项目资助
关键词
丝胶
2型糖尿病
肝脏
胰岛素受体(IR)
血糖
Sericin
Type 2 diabetes mellitus
Liver
Insulin receptor (IR)
Blood glucose