高效液相色谱法同时测定苯巴比妥、苯妥英钠、卡马西平血药浓度

Determination of Phenobarbital,Phenytoin and Carbamazepine in Human Serum of Epileptic Children by HPLC

目的：建立高效液相色谱（HPLC）法同时测定癫痫患儿血浆中苯巴比妥（PB）、苯妥荚钠（PH）、卡马西平（CBZ）三种药物浓度。方法：采用HypersllODS2（150mm×4．6mm，5μm）色谱柱，流动相甲醇一水（54：46），流速0．9mlMmin，检测波长240nm，柱温25qc，进样量10μL。结果：PB、PH、CBZ的线性范围分别为1．04～104．24μg/L、0．91—67．99μg／mL、0．37—37．00μg／mL，提取回收率分别为91．50％、89．20％、95．80％。结论：本研究所建立的方法操作简便、结果准确、重现性好，适用于同时测定PB、PH、CBZ三种药物的血药浓度。

Objective ： To establish a HPLC method for simultaneous determination of phenobarbital （ PB ）, phenytoin （ PH ） and carbamazepine （CBZ） concentrations in human serum of epileptic children. Methods： The separation was performed on a Hypersil ODS2（ 150 mmx4. 6 ram, 5 μm）column. The mobile phase was methanol-water（ 54 ： 46） at a flow rate of 0. 9 mL/min, the column was at 25 ℃, and detected at 240 nm, the injection volume was 10 μL. Results： The calibration curves were linear within the range of 1.04 - 104. 24 μg/mL for PB, 0. 91 ~ 67.99 μg/mL for PH, 0. 37 ~ 37.00 μg/mL for CBZ, and the average recoveries were 91.50%, 89.20%, 95. 80% respectively. Conclusion： This method is simple, accurate, reproducible and suitable for the determination of PB, PH, CBZ blood concentrations of three drugs.