人参皂苷Rg3对人鼻咽癌CNE-2细胞血管生成拟态及迁移能力的影响

20(R)-ginsenoside Rg3 inhibits vasculogenic mimicry and cell migration of human nasopharyngeal carcinoma CNE-2 cell line in vitro

目的探讨人参皂苷Rg3对人低分化鼻咽癌细胞系CNE-2体外血管生成拟态及迁移能力的影响。方法用不同浓度(0、38、76和114μmol/L)的Rg3处理CNE-2细胞后,体外管道形成抑制实验检测Rg3对CNE-2细胞管道形成能力的影响;Transwell小室法检测Rg3对CNE-2细胞迁移能力的影响;Western blot法检测Rg3对CNE-2细胞中COX-2、HIF-1α、VEGF和Fascin1蛋白表达的影响。结果人参皂苷Rg3能抑制CNE-2细胞体外管道形成的能力,其管状结构数量与Rg3浓度呈负相关(P<0.01);Rg3浓度大于76μmol/L时可以抑制CNE-2细胞的迁移能力,与0μmol/L Rg3比较差异有统计学意义(P<0.05);且Rg3对COX-2、HIF-1α、VEGF和Fascin1蛋白表达的影响趋势分别与Rg3对血管生成拟态和迁移能力的变化趋势呈正相关(P<0.05)。结论人参皂苷Rg3能抑制CNE-2细胞体外血管生成拟态及迁移能力,其分子机制可能与抑制CNE-2细胞中COX-2、HIF-1α、VEGF和Fascin1蛋白的表达有关。

Objective To study the inhibitory effects of 20（ R）- ginsenoside Rg3 on vasculogenic mimicry and migration of human nasopharyngeal carcinoma CNE- 2 cell line in vitro. Methods CNE- 2 cells treated with different concentrations of Rg3（ 0 μmol·L^-1,38 μmol·L^-1,76 μmol·L^-1 and 114 μmol·L^-1） were assayed with transwell assay and anti- angiogenic test for testing the potential of migration and tube- like structure（ TLSs） formation,respectively. Meanwhile,the expression of COX- 2,HIF- 1α,VEGF and Fascin1 proteins were detected by Western blotting.Results The formation of TLSs in CNE- 2 cells were significantly inhibited by Rg3 in concentration dependent manners（ P〈0. 01）; so was the migration ability of CNE- 2 cells（ P〈0. 05）. At the same time,the expression of COX- 2,HIF- 1α,VEGF and Fascin1 in CNE- 2 cells were down- regulated by Rg3. A significantly positive correlation between the expression of Fascin1 and the migration ability was observed（ P〈0. 05）,so was the significantly positive correlation between the expression of COX- 2,HIF- 1α and VEGF and the inhibition of the TLS formation（ P〈0. 05）. Conclusion Rg3 can inhibit the vasculogenic mimicry and migration of CNE- 2 cells in vitro,via the down- regulation of COX- 2,HIF- 1α,VEGF and Fascin1.