刺梨黄酮的精制及其抗氧化活性比较

Refining and antioxidant activity of flavonoids from Rosa roxburghii Tratt.

以8种大孔树脂为筛选对象,研究大孔树脂对刺梨黄酮的吸附和解吸情况,并以芦丁标准品为对照,考察刺梨黄酮精制前后的抗氧化活性。结果表明:AB-8大孔树脂适用于刺梨黄酮的精制;经AB-8大孔树脂静态吸附精制后,刺梨黄酮质量分数达37.24%,为粗提物的4.4倍。抗氧化活性研究表明,刺梨黄酮的抗氧化活性与质量浓度之间有量效关系,且精制后刺梨黄酮的抗氧化活性显著高于粗提物。因此,刺梨黄酮有一定的抗氧化活性,可作为一种新型的天然抗氧化剂用于保健食品、化妆品和生物制药等行业。

The adsorption characteristics of flavonoids from Rosa roxburghii Tratt. （ FRR） was investigated using eight kinds of macroporous resin, and the antioxidant activity of flavonids before and after refining was examined using rutin as the control in the research. The results showed that AB-8 macroporous resin was the best resin for FRR refining. After refining, the contents of FRR reached more than 37.24%, was the 4.4 times than before. The antioxidant activity data showed the antioxidant activity of FRR were linear with its mass concentration, and the antioxidant activitiy of FRR after refining was higher than FRR before refining. Flavonoids from FRR had strong antioxidant activities and could be used in healthy food, cosmetic and medicine.