中药白英的高效液相色谱指纹图谱研究

Research of HPLC finger prints on Solanum lyratum Thunb

目的:建立不同产地白英药材的HPLC指纹图谱,为建立白英药材的质量标准提供依据。方法:采用Waters C18(150 mm×4.6 mm,5μm)(柱前加保护柱)色谱柱;流动相:乙腈-0.2%甲酸水溶液,梯度洗脱;流速:1.0 m L/min;检测波长:285 nm;柱温:室温。结果:通过对各个产地白英的HPLC指纹图谱进行直观分析,并采用中药色谱指纹图谱相似度软件进行相似度评价,经过比较,确定了13个峰作为特征峰,由此建立了白英的HPLC指纹图谱。结论:该法所得图谱清晰,特征直观,所建立的白英药材的HPLC指纹图谱可作为评价其质量的参考。

Objective：To establish HPLC finger prints of Solanum lyratum Thunb. of different areas and provide the basis for quality standard. Methods：Waters C18 （150 mm ×4.6 mm,5 μm） column was as chromatographic column,and mobile phase was acetonitrile-0.2% formic acid （gradient elution）. Flow rate was 1.0 mL/min and detection wavelength was 285 nm. Column temperature was the room temperature. Results：HPLC finger prints of Solanum lyratum Thunb.of different ar-eas were analyzed and their similarities were evaluated through TCM fingerprint similarity evaluation softwar. 13 peaks in the spectrum were determined as characteristic peak of Solanum lyratum Thunb.. Conclusion：The spectrum is clear and could be used as the basis of quality evaluation.