摘要
目的观察氧化再生纤维素防粘连膜(Interceed)治疗新西兰大白兔宫腔粘连的疗效和安全性。方法采用机械和感染双重损伤法构建新西兰大白兔宫腔粘连动物模型。将48只雌兔随机分为4组:正常对照组、Interceed对照组、模型组、Interceed治疗组。正常对照组:行假手术,不造模。Interceed对照组:正常兔宫腔内放置Interceed;模型组、Interceed治疗组采用机械和感染双重损伤法构建宫腔粘连动物模型。模型组:建模后不作治疗;Interceed治疗组:建模后1周宫腔内放置Interceed。每组分别于治疗前(建模后1周)、治疗后2周和4周各处死4只,收集双侧子宫组织,HE染色观察子宫内膜形态及腺体计数;Masson染色检测子宫内膜纤维化程度;免疫组化法检测子宫内膜转化生长因子β1(TGF-β1)的蛋白表达。结果 (1)Interceed对照组各时间点子宫内膜腺体数量、纤维化面积比率、TGF-β1蛋白表达与正常对照组比较差异均无统计学意义(P>0.05)。(2)在治疗前,模型组和Interceed治疗组子宫内膜腺体数量均低于正常对照组(P<0.001),而纤维化面积比率和TGF-β1蛋白表达均高于正常对照组(P<0.001)。(3)在治疗后2、4周,Interceed治疗组TGF-β1蛋白表达均高于正常对照组(P<0.001),而腺体数量、纤维化面积比与正常对照组比较差异均无统计学意义(P>0.05);Interceed治疗组子宫内膜腺体数量、纤维化面积比率、TGF-β1蛋白表达与模型组比较差异均有统计学意义(P<0.001)。结论 Interceed可以有效治疗新西兰大白兔宫腔粘连,并且对新西兰大白兔子宫内膜无明显不良影响。
Objective To observe the effect and safety of oxidized regenerated cellulose on the treatment of intrauterine adhesion in New Zealand white rabbits.Methods The intrauterine adhesions rabbit model was established by mechanical injury and infection double-injury method.Forty-eight female rabbits were divided into 4 groups：normal control group,Interceed control group,model group,and Interceed therapy group;in which the rabbits received sham operation,Interceed placement,modeling alone or modeling with Interceed treatment,respectively.Interceed placement was performed 1 week after modeling.Each 4 rabbits were sacrificed in each group before treatment（ 1 week after modeling）,and 2 and 4 weeks after treatment.The uterine tissues were collected.HE staining was performed for endometrial morphologic observation and gland count.Masson staining was performed to detect the degree of endometrial fibrosis.The expression of TGF-β1protein was detected by immunohistochemistry.Results（ 1） There was no significant difference between the normal control group and the Interceed control group in the endometrial gland count,the ratio of fibrosis area or the expression of TGF-β1protein（ P 〉0.05）.（ 2） Before the treatment,the endometrial gland counts in the model group and Interceed therapy group were significantly lower than that in the normal control group（ P〈0.001）,while the ratio of fibrosis area and the expression of TGF-β1protein were significantly higher than those in normal control group（ P〈0.001）.（ 3） Two and 4 weeks after treatment,the expression of TGF-β1protein in the Interceed therapy group was significantly higher than that in normal control group（ P〈0.001）,while there was no significant difference in the endometrial gland count or the ratio of fibrosis area between the Interceed therapy group and normal control group（ P〉 0.05）.However,there were significant differences in endometrial gland count,the ratio of fibrosis area and the expression of TGF-β1 protein between the Interceed therapy group and the model group（ P〈0.001）.Conclusion The Interceed treatment for intrauterine adhesion is effective and safe,without obvious adverse reactions on the endometrium in New Zealand whiterabbit.
出处
《广东医学》
CAS
北大核心
2015年第10期1469-1474,共6页
Guangdong Medical Journal
基金
国家自然科学基金资助项目(编号:81270658)
