摘要
以藏鸡为研究对象,根据NCBI上登录的原鸡A-FABP和H-FABP基因序列设计引物,利用RT-PCR方法克隆藏鸡A-FABP和H-FABP基因的CDS区,并进行生物信息学分析.结果表明:藏鸡A-FABP和H-FABP基因的ORF分别为399 bp、402 bp,编码132、133个氨基酸;A-FABP和H-FABP分子量分别为14.91ku、14.84ku,等电点分别为6.34、5.92,均为亲水蛋白,无信号肽和跨膜结构.磷酸化位点分别有9、6个,O糖基化位点分别有10、5个;藏鸡A-FABP和H-FABP的氨基酸序列与原鸡的同源性最高,分别为99%、100%,在鸟类动物中序列比较保守.
Tibetan chicken was used as tile experimental animal. PCR primers were designed according to chicken A-FABP and H-FABP gene sequences in NCBI. RT-PCR technique was adopted to amplify the A-FABP and H-FABP gene and the sequences were analyzed by bioinformatics methods. The results showed that the cDNA sequences of Tibetan chicken A-FABP and H-FABP gene were 399 bp and 402 bp,encoding 132 and 133 amino acids. The molecular weights of A-FABP and H-FABP protein were 14. 91ku and 14. 84ku, and theoretical isoelectric points were 6. 34 and 5.92. They were both hydrophilic proteins without signal peptides and transmembrane structure. There were 9 phosphorylation sites, 100-glycosylation sites within the A-FABP protein, while there were 6 phosphorylation sites and 50-glycosylation sites within the H-FABP protein. The amino acid sequences of Ti- betan chicken A-FABP and H-FABP Showed the highest homologous with G allus gallus among the animals assayed,up to 99% and 100% respectively,and both is highly conserved in birds.
出处
《西南民族大学学报(自然科学版)》
CAS
2015年第3期270-276,共7页
Journal of Southwest Minzu University(Natural Science Edition)
基金
四川省畜禽育种攻关项目(2011NZ0099-6)
西南民族大学项目"四川民族地区主要地方鸡种遗传资源研究"(2012NFW001)