嗜水气单胞菌胁迫下东北林蛙皮肤MyD88和TRAF6基因表达的动态变化

Expression Dynamics of MyD88 and TRAF6 Genes in Rana dybowskii Infected with Aeromonas hydrophila

采用高通量测序获得东北林蛙(Rana dybowskii)MyD88和TRAF6mRNA序列设计引物,将蛙类易感微生物嗜水气单胞菌(Aeromonas hydrophila,Ah)经东北林蛙腹腔注射后,用荧光定量PCR技术分析蛙皮肤MyD88和TRAF6mRNA表达水平的变化,以期揭示东北林蛙在识别嗜水气单胞菌后,其机体TLR信号通路中相关分子的免疫效应。数据显示,处理后12h试验组皮肤MyD88和TRAF6mRNA的表达量与对照组相比有明显升高且达到峰值,分别为2.38倍和8.12倍,其中TRAF6mRNA表达水平尤为显著,在24h^48h仍处于较高水平,分别为对照组的4.32倍和2.54倍。结果表明,嗜水气单胞菌胁迫能促使东北林蛙机体TLR信号通路中的MyD88和TRAF6mRNA的表达量上调。本研究探讨了微生物侵袭时东北林蛙的TLR信号调控机制,为认识两栖类机体的先天免疫系统奠定了一定的理论基础。

Primers of MyD88 and TRAF6 mRNA sequences were designed by high-throughput sequencing of Rana dybowskii . The real-time PCR was used to detect the expression levels of MyD88 and TRAF6 mR- NA in Rana dybowskii skin infected with Aeromonas hydrophila in order to reveal the immune effects of TLR signaling pathway associated molecules. Data showed that 12 h after infection the experimental group MyD88 and TRAF6 mRNA expression levels had significantly increased by 2.38 fold and 8.12 fold com- pared with the control group and reached peaks, TRAF6 mRNA expression levels was the most significant and remained at a high level at 24 h-48 h . The study indicated Aeromonas hydrophila induced Rana dy- bowskii skin TLR signaling pathways and upregulated MyD88 and TRAF6 mRNA expression. We investi- gated the regulation mechanism of Rana dybowskii TLR signaling after microbial invasion, which provide theoretical basis for understanding amphibians＇ innate immune system.