Fugu xanthopterus与Fugu bimaculatus及其正反交子一代的ISSR遗传分析

Genetic analysis with ISSR method on reciprocal hybrid progeny between Fugu xanthopterus and Fugu bimaculatus

本研究开展了黄鳍东方鲀(Fugu xanthopterus)和双斑东方鲀(Fugu bimaculatus)人工正反杂交实验,通过ISSR分子标记技术进行遗传分析,结果表明:亲本及其正反交子一代个体间都有其特异的ISSR分子标记,亲本群体的遗传多样性指数(H')分别为0.314 8和0.276 5,正反交子代群体的遗传多样性指数分别为0.336 2和0.325 7,黄鳍东方鲀和双斑东方鲀群体间的遗传距离(D)较远为0.895,反交群体与双斑东方鲀群体间的遗传距离最近为0.169.实验数据表明双斑东方鲀对杂交子代的影响更为显著.

In this study artificial crossbreeding between Fugu xanthopteru and Fugu bimaculatus was made.The In-ter-Simple Sequence Repeats （ISSR）technology was applied to analyze the genetic diversity between Fugu xan-thopterus,Fugu bimaculatus and their hybrid progeny （F1）.The results showed that there were some particular IS-SR loci among Fugu xanthopterus,Fugu bimaculatus and their reciprocal hybrids.The genetic diversity index （H′） of parent was 0.314 8 and 0.276 5 while between their reciprocal hybrids was 0.336 2 and 0.325 7.The genetic distance （D）between Fugu xanthopterus and Fugu bimaculatus was 0.895 while the genetic distance between the reversed hybrid population and Fugu bimaculatus was 0.169.This experiment indicated that the effect of Fugu bi-maculatus on hybrid progeny was much more significant than Fugu xanthopterus.