HPLC-DAD法同时测定肝八味胶囊中4种成分的含量

Simultaneous determination of four ingredients in Gan Bawei capsule by HPLCDAD

目的：用高效液相色谱（HPLC）法同时测定肝八味胶囊中4种成分的含量。方法采用 HPLC法，色谱柱为C18柱（250 mm×4.6 mm ，5μm），以乙腈为流动相A ，以水为流动相B ，梯度洗脱；检测波长为240 nm ；流速1.0 ml／min。结果丹酚酸B对照品在15.026∽100.17μg／ml范围内呈良好线性关系，平均加样回收率为96.6％（n＝6），RSD＝1.8％（n＝6）；芍药苷对照品在14.335～95.564μg／ml范围内呈良好线性关系，平均加样回收率为97.0％（n＝6），RSD＝1.7％（n＝6）；虎杖苷对照品在8.235～54.90μg／ml范围内呈良好线性关系，平均加样回收率为97.2％（n＝6），RSD＝1.8％（n＝6）；大黄素对照品在1.5825～10.55μg／ml范围内呈良好线性关系，平均加样回收率为98.1％（n＝6），RSD＝1.7％（n＝6）。结论此法简单准确、重现性好、专属性强、阴性对照无干扰，适用于肝八味胶囊的质量控制。

Objective To establish an HPLC-DAD method for simultaneous determination of four ingredients in Gan Ba-wei capsule .Methods HPLC method was adopted with C18 column（250 mm × 4.6 mm ,5 μm） ,Acetonitrile as mobile phase A and water as mobile phase B in gradient elution mode . The detection wavelength was 240 nm and the flow rate was 1.0 ml/min .Results Salvianolic acid B reference substance was excellently linear in the range of 15.026∽100.17 μg/ml ,the average recovery was 96.6% （n=6）and RSD was 1.8% （n=6） .Paeoniflorin reference substance was excellently linear in the range of 14.335~95.564 μg/ml ,the average recovery was 97.0% （n=6）and RSD was 1.7% （n=6） .Polydatin reference sub-stance was excellently linear in the range of 8.235-54.90 μg/ml ,the average recovery was 97.2% （n=6）and RSD was 1.8%（n=6） .Emodin reference substance was excellently linear in the range of 1.582 5-10.55 μg/ml ,the average recovery was 98.1% （n=6）and RSD was 1.7% （n=6） .Conclusion The method was easy ,accurate ,reproducible ,specific and without in-terference in negative control ,which could be used for quality control of Gan Bawei capsule .