植物蛋白质双向电泳样品制备研究进展

Research Progress on the Preparation of Samples of Plant Protein for Two-Dimensional Electrophoresis

蛋白质双向电泳是蛋白质组学的支撑技术,在微生物和动物蛋白质分析上取得较大进展,但在植物蛋白质分析上却进展不大,究其原因在于难以制备到适于双向电泳的植物蛋白质样品。笔者综述了植物蛋白质双向电泳样品制备的方法,分析各种方法的优缺点,并提出今后植物蛋白质样品制备的研究重点。

Two-dimensional electrophoresis is the fundamental technology of proteomics, it makes great progress in protein analysis of animals and microbes. However, it makes little progress in protein analysis of plants due to the hardness of the preparation of plant protein＇s samples suited for two-dimensional electrophoresis. In this paper, methods for the preparation of plant protein＇s samples were reviewed, merits and drawbacks of these methods were analyzed, and the research focus on the preparation of plant protein＇s samples in the future was putted forward.