二妙散对兔膝关节创伤性滑膜炎IL-6和TNF-α的影响

Effects of Ermiao San on rabbit traumatic knee joint inflammation synovial fluid IL-6 and TNF-α

目的:探讨二妙散对兔膝关节创伤性滑膜炎的疗效机制。方法:将36只日本大耳兔随机分为3组,空白组、模型组、受试组各12只,创建创伤性膝滑膜炎动物模型,从造模第1天起开始,空白组及模型组予以0.9%氯化钠溶液灌服,受试组予以二妙散灌服,第11天观察膝关节周径改变、体表温度变化;提取关节液,用酶联免疫吸附测定法检测各组关节液中IL-6和TNF-α水平情况。于髌股关节交界处取滑膜组织1块,标本行石蜡切片,观察滑膜组织的病理切片。结果:与空白组比较,模型组膝关节周径、体表温度、关节液IL-6及TNF-α表达显著增加(P<0.01,P<0.05);与模型组比较,受试组关节液IL-6及TNF-α表达显著降低(P<0.01,P<0.05)。结论:二妙散对兔膝创伤性滑膜炎有较好的治疗作用;二妙散能通过抑制IL-6和TNF-α发挥对创伤性滑膜炎的治疗作用。

Objective： To explore the mechanism of Ermiao San on rabbit traumatic knee joint inflammation. Methods： 36 rabbits were randomly divided into three groups, with 12 rabbits in each group. The control group and model group were treated with saline gavage, and the test group was treated with Ermiao San. Joint circumference changes and joint surface temperature changes were observed on day 11, and then animals were sacrificed to extract the synovial fluid, using enzyme-linked immunosorbent assay to detect the rabbit knee joint fluid IL-6 and TNF-α levels. The synovial tissue was taken from the junction of the patella and femur. After the process of paraffin section, pathological section of the synovial tissue biopsy was observed. Results： Compared with the blank group, knee circumference, surface temperature of the model group, IL-6 and TNF-α expression of the joint fluid increased significantly （P〈0.01, P〈0.05）; Compared with model group, IL-6 and TNF-α expression of the joint fluid decreased significantly （P〈0.01, P〈0.05）. Conclusion： Ermiao San has a positive therapeutic effect on the rabbit knee joint traumatic synovitis. Ermiao san can inhibit IL-6 and TNF-α levels to treat traumatic synovitis.