不同色谱条件下测定奥美拉唑含量方法的比较研究

Comparative Study on the Different Chromatographic Conditions of Determining Omeprazole Content

目的比较2种不同色谱条件下用高效液相色谱法检测奥美拉唑含量的差异。方法建立C18-甲醇体系与C8-乙腈体系,并分别测定3个不同厂家奥美拉唑的含量,对含量测定结果进行统计学分析。结果 C18-甲醇体系与C8-乙腈体系测定三家不同厂家奥美拉唑的含量,差异均无统计学意义(P>0.05)。结论 2种不同色谱条件下对奥美拉唑进行含量测定,其测定方法无统计学差异。

Objective To compare the content of omeprazole under differences chromatographic conditions for HPLC between two different methods. Methods The C18-methanol system and C8-acetonitrile system were established,and then used the systems to determinate the contents of omeprazole from 3 different manufacturers. Results The content determination of omeprazole from three different manufacturers were determined by C18-methanol methanol and C8-acetonitrile system. There were no significant differences between them（P〉0.05）. Conclusion The two kinds of different chromatographic conditions for determining omeprazole have no significant difference.