沉默乙酰肝素酶调控血管内皮生长因子C对胰腺癌细胞恶性生物学行为的影响

Heparanase regulates VEGF-C expression in vitro and its effect on pancreatic ductal cell adenocarcinoma

目的 检测乙酰肝素酶（HPSE）基因与血管内皮生长因子C（VEGF-C）基因在胰腺导管细胞癌组织中的表达,构建含HPSE基因的质粒及针对HPSE基因的小干扰RNA（siRNA）,体外观察其对胰腺癌BxPC-3细胞系VEGF-C表达水平及恶性生物学行为的影响.方法 RT-qPCR检测34例胰腺癌组织中两种基因的相对表达水平,分析其与临床病理特征的关系.设计并构建重组质粒GV230/HPSE及HPSE-siRNA,脂质体介导法转染BxPC-3细胞,RT-qPCR及Western blot检测HPSE及VEGF-C的表达状况,Transwell侵袭实验及细胞划痕实验观察转染细胞体外侵袭迁移能力的改变.结果 Spearman秩相关分析显示胰腺癌组织HPSE与VEGF-C的mRNA水平呈正相关（r=0.812,P＜0.01）,HPSE与VEGF-C的表达与肿瘤的分化程度、淋巴结转移及TNM分期显著相关（P＜0.01）.RT-qPCR与Western blot结果显示GV230/HPSE组与空白对照组相比HPSE及VEGF-C的mRNA及蛋白水平均增高（P＜0.01）,HPSE-siRNA组两种基因的mRNA及蛋白的水平均降低（P＜0.01）.Transwell侵袭实验及细胞划痕实验中过表达组的侵袭能力增强（P＜0.05）,干扰组细胞的侵袭能力被抑制（P＜0.05）. 结论 联合检测HPSE mRNA与VEGF-C mRNA有助于指导胰腺癌临床治疗及判断预后.HPSE可调控胰腺癌细胞VEGF-C的表达,并提高其体外侵袭迁移能力,提示HPSE可作为一个胰腺肿瘤治疗潜在的新靶点.

Objective To measure HPSE and VEGF-C expression in pancreatic cancer tissues and to observe the effect of small interfering RNA targeting heparanase gene on VEGF-C expression and malignant biological behavior of pancreatic cancer cell lines BxPC-3.Methods We measured the relative mRNA levels of HPSE and VEGF-C in 34 pancreatic ductal cell adenocarcinoma（PDAC） specimens by RT-qPCR.The recombinant plasmid GV230/HPSE was constructed.BxPC-3 was transiently transfected with GV230/HPSE and HPSE-siRNA.The expression levels of HPSE and VEGF-C were measured by performing florescent real-time quantitative PCR （RT-qPCR） and immunoblotting.The invasion and migration potential of treated BxPC-3 was evaluated by transwell invasion assay and Scratch-wound assay.Results Spearman rank correlation analysis indicated a positive correlation between HPSE and VEGF-C （r =0.812,P 〈0.01）in pancreatic cancer tissues.HPSE and VEGF-C overexpression was correlated with tumor differentiation,lymph node metastasis and TNM stage（all P 〈0.01）.RT-qPCR and Western blot showed high expression of HPSE mRNA and VEGF-C mRNA in GV230/HPSE group than that in control group,compared to low expression of HPSE mRNA and VEGF-C mRNA in siRNA group （all P 〈 0.01）.Invasion chamber assay and Scratch-wound assay showed higher invasion in GV230/HPSE group than that in control group,while lower invasion in siRNA group （all P 〈 0.01）.Conclusions Joint detection of HPSE and VEGF-C may be of significance in predicting prognosis of patients with pancreatic cancer.HPSE regulates the expression of VEGF-C and facilitates invasion and migration of BxPC-3 in vitro.