一种输尿管支架管纳米铁牵引线的抗张强度及生物相容性研究

Biocompatibility and tensile strength of a nanoscale iron traction suture for ureteral stent

目的评价一种新型输尿管支架管纳米铁牵引线的抗张强度及生物相容性。方法分别对纳米铁牵引线的抗张强度及用膀胱镜拔除普通输尿管支架管的拔管阻力进行测量并分析比较。在SD大鼠背部脊柱左侧肌肉组织中植入普通手术缝线作为对照组,在SD大鼠脊柱右侧肌肉组织中埋入纳米铁牵引线作为实验组,分别于术后1、2、4、6、8、12周取标本,HE染色,光镜观察,根据软组织植入物生物学定级评分方法对两组植入物所引起的组织学反应进行反应灶厚度定量、反应灶定性及接触面定性评分,并对得分进行对比分析。结果纳米铁牵引线的抗张强度显著大于输尿管支架管拔管阻力(P<0.05)。术后两组植入丝线周围组织都表现出典型的异物慢性炎症反应,组织学可见两组植入丝线周围都以单核巨噬细胞系统浸润为主,并可见吞噬丝线纤维或纳米铁颗粒的异物巨细胞。生物学定级评分比较结果显示,术后1周时两组间反应灶厚度定量评分及接触面定性评分差异有统计学意义(P<0.05),其余各时期评分两组间无明显差异(P>0.05)。结论纳米铁牵引线具有良好的抗张强度和组织相容性。

Objective To evaluate the tensile strength and biocompatibility of a novel nanoscale iron traction suture for ureteral stent. Methods The tensile strength of the suture and the resistance of removing common ureteral stent by cystoscopy were measured and compared. Then the nanoscale iron traction suture was implanted into the muscle tissue on the right side of the spine on the back of SD rats （ as experimental group）, while the common surgical suture was implanted on the left side （as control group）. Specimens were taken at 1, 2, 4, 6, 8 and 12 weeks after implantation, stained with hematoxylin and eosin, and observed by light microscopy. A histological grading scale was used to evaluate the tissue reaction to the 2 implants by examining the thickness and quality of the reaction capsule surrounding the implants, and the quality of the interface. The results were analyzed between the 2 groups. Results The tensile strength of the nanoscale iron traction suture was significant greater than the resistance of removing ureteral stent （ P 〈 0.05 ）. The surrounding tissues of the both implants showed a typical chronic inflammatory reaction caused by foreign body. The 2 implants were surrounded by mononuclear phagocyte infiltration, and some foreign body giant cells were seen which had devoured the suture or the nanoscale iron suture. The histological grading showed there was statistical different between the 2 groups on the thickness of the reaction capsule surrounding the implants and the quality of the interface at 1 week after implantation （P 〈0.05）, but no significant differences were seen in the histological score between the 2 groups at the other periods （P 〉 0.05）. Conclusion Thenanoscale iron traction suture possesses good tensile strength and biocompatibility.