果蝇卵巢滤泡细胞谱系发育中的功能性microRNA筛查

A Screening for Functional micro RNA in the Development of Drosophila Ovarian Follicle Cell Lineage

在果蝇发育过程中,micro RNA(mi RNA)作为负调控因子起着重要的作用。该文旨在研究micro RNA在果蝇卵巢滤泡细胞谱系中的功能,该细胞谱系因易于体内遗传操作而成为研究细胞命运决定与细胞迁移机制的良好模型。为了确认此过程中的功能性mi RNA,作者利用UAS/GAL4二元表达系统对31个果蝇mi RNA进行了表型筛选。结果表明,若干mi RNA可以在卵子发生过程中引起多种严重表型。过表达与敲减mi R-7均能阻断边界细胞迁移。mi R-1、mi R-124和mi R-263b则在茎细胞诱导、边界细胞迁移或卵壳图式中发挥功能。结果表明,该文所用基于UAS/GAL4的方法可用于确认mi RNA的功能。

As negative regulators of gene expression, microRNA （miRNA） plays important roles during Drosophila development. In this paper, we aimed to investigate the effects of miRNA on the commitment of Droso- phila ovarian follicle cell lineage, which is an in vivo genetically tractable model to understand the mechanisms of cell fate determination and cell migration. To identify functional miRNA in the process, we performed a phenotypic screening for total 31 Drosophila miRNA by UAS/GAL4 binary expression system. The results showed that several miRNA cause multiple severe defects during oogenesis. And both the overexpression and knock-down ofmiR-7 could block the border cell migration, miR-1, miR-124 and miR-263b may function on stalk cell induction, border cell migration and egg shell patterning. The results demonstrated that UAS/GAL4 system-based strategy used in this paper was feasible for functional miRNA identification.