应用Label-free技术研究人牙囊细胞和牙周膜成纤维细胞的差异蛋白质

The differential proteomics of human dental follicle cells and human periodontal ligament fibroblasts analysed with Label-free technology

目的:应用Label-free蛋白定量技术研究牙囊细胞(h DFCs)和牙周膜成纤维细胞(h PDLFs)差异表达蛋白质,为发现h DFCs向h PDLFs转化过程中的重要蛋白质提供参考。方法:应用Label-free蛋白定量技术,结合ultramate 3000 nano-UPLC软件,对h DFCs和h PDLFs总蛋白提取液进行蛋白定性定量检测。然后分别对蛋白质的鉴定结果行重复性、关联性、蛋白相对含量和差异表达蛋白等进行分析;对总体蛋白质和差异表达蛋白质行生物信息学GO或KEGG分析。结果:h DFCs和h PDLFs蛋白相对含量及总体蛋白GO分析结果相似。定量分析显示,当h DFCs分化形成h PDLFs后,有22个差异表达蛋白质,其中下调蛋白15个(P<0.05,FC>2),上调蛋白7个(P<0.05,FC>2)。结论:h DFCs和h PDLFs蛋白质表达谱相似;22个差异表达蛋白质为研究h DFCs向h PDLFs的转化机制提供了方向。

AIM： To identify the differentially expressed proteins of human dental follicle cells（ h DFCs） and human periodontal ligament fibroblasts（ h PDLFs） with Label-free protein quantification technique. METHODS： Combined with ultramate 3000 nano-UPLC software,Label-free protein quantification was used to examine the total protein extraction samples from h DFCs and h PDLFs. For the proteins identified,the repeatability,the correlation,the relative content of proteins and the differentially expressed proteins of h DFCs and h PDLFs were analyzed. Bioinformatic analysis of GO and KEGG was applied for the analyses of total proteins of h DFCs and h PDLFs,as well as the differentially expressed proteins. RESULTS： The relative content of proteins and the results of GO analysis of h DFCs swere similar to those of h PDLFs. 22 differentially expressed proteins were identified,of which 15 were down-regulated,7 were up-regulated. CONCLUSION： Label-free quantitative proteomics confirmed the similar proteins profile between h DFCs and h PDLFs. 22 differentially expressed proteins provide clues for the transformation mechanism from h DFCs to h PDLFs.