沉默STAT1基因增强放射抗拒鼻咽癌CNE-2R细胞放射敏感性

Silencing STAT1 gene enhances radiosensitivity of radioresistant human nasopharyngeal carcinoma CNE-2R cells

目的 研究基因STAT1沉默对人放射抗拒鼻咽癌细胞放射敏感性的影响.方法 慢病毒介导的STAT1基因转染放射抗拒鼻咽癌细胞CNE-2R,荧光定量RT-PCR技术检测沉默效果.MTT法检测转染前后细胞增殖活性,流式细胞技术检测细胞周期及凋亡,克隆形成实验检测转染前后细胞放射敏感性变化.结果 慢病毒转染后放射抗拒鼻咽癌细胞STAT1表达降低（F=429.87,P＜0.05）、细胞生长抑制（F=3.88 ～4.63,P＜0.05）、凋亡率增加（F =38.13,P＜0.05）、放射敏感性增加（F=252.80,P＜0.05）、细胞周期中G0/G1、S和G2/M期未见明显差异（P＞0.05）.结论 沉默STAT1基因能增加放射抗拒鼻咽癌细胞CNE-2R的放射敏感性.

Objective To study the effect of silencing STAT1 on radiation sensitivity of nasopharyngeal carcinoma cells.Methods Lentivirus-mediate STAT1 gene-transfer was performed to radioresistant human nasopharyngeal carcinoma CNE-2R cells and the transfection efficiency was detected by RT-PCR.The cell proliferation was measured by MTT,and the apoptosis was analyzed by flow cytometry.The radiosensitization ratio was measured according to the clone formation efficiency.Results After transfection with STAT1 siRNA,the expression of STAT1 was effectively inhibited in CNE-2R cells （F =429.87,P 〈 0.05）,the cell proliferation was decreased （F =3.88-4.63,P 〈 0.05）,the apoptosis was increased （F =38.13,P 〈 0.05）,the cell radiosensitivity was enhanced （F =252.80,P 〈 0.05）,but the cell cycle distribution at G0/G1,S and G2/M were not influenced （P 〉 0.05）.Conclusions Silencing STAT1 could increase radiosensitization of nasopharyngeal carcinoma cells.